单词 | electroporate |
释义 | electroporatev. Biochemistry. transitive. To apply high-voltage pulses of electricity to (cells or protoplasts) in order to create temporary pores in the cell membranes. Also: to facilitate the introduction of (foreign material, esp. DNA or RNA) into a cell or protoplast using this technique. ΚΠ 1985 Proc. National Acad. Sci. U.S.A. 82 5827/1 Protoplasts were washed and electroporated with a 300V, 54-msec pulse in each solution. 1987 Nucleic Acids Res. 15 5823 (title) Expression of mRNA electroporated into plant and animal cells. 1993 Cell 73 251/1 The targeting vector..was electroporated into E14 ES cells..that were subsequently cultured for 8 day. 2006 Drug Discov. Today 11 1069/2 Higher electric fields can be used to carry out automated wound healing assays and..to electroporate cells. Derivatives eˈlectropoˌrated adj. (of a cell or protoplast) that has been treated by electroporation. ΚΠ 1985 Trends Biotechnol. 3 191 (title) Transient gene expression in electroporated plant cells. 2007 Gene 386 176/1 Electroporated cells were cultured onto selective LB plates. This is a new entry (OED Third Edition, June 2008; most recently modified version published online March 2022). < v.1985 |
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