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单词 gram stain
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Gram stain
1. As a modifier (also in the genitive). Designating a method of staining used to identify and classify bacteria and certain other microorganisms, esp. in Gram stain.The technique of Gram staining involves several steps: an initial staining with a purple dye (gentian violet), fixation of the colour with an iodine solution, washing with alcohol or acetone, and a final staining with a red dye. Some microorganisms retain the purple dye and are known as Gram-positive; Gram-negative organisms lose the purple colour and are stained by the red dye. These staining properties are determined mainly by the structure and peptidoglycan content of the microorganism's cell wall, and correlate with the response of the microorganism to certain types of antibiotic.
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the world > life > biology > laboratory analysis > processes > [noun] > using stains or dyes
overstaining1880
Gram stain1884
Nissl method1895
osmication1899
counterstaining1901
cytophotometry1952
polychroming1958
immunofluorescence1960
the world > life > biology > laboratory analysis > material > [noun] > staining material
osmium tetroxide1869
picrocarmine1872
rose bengal1878
stain1880
erythrosin1884
Gram stain1884
vesuvin1885
Nile blue1888
pyronin1895
Janus green1898
counterstain1899
Nissl stain1899
Leishman stain1904
trypan blue1911
quinacrine mustard1957
1884 Edinb. Clin. & Pathol. Jrnl. 12 July 804 Dr. M. Afanassiw of St. Petersburg..recommends the following modification of Dr. Gram's method.]
1884 Brit. Med. Jrnl. 6 Sept. 487/2 Gram's method gives good results with many bacteria.
1888 Lancet 6 Oct. 684/2 The organs and blood, after being treated with Gram's stain, were found to contain cocci.
1906 Jrnl. Infectious Dis. 3 495 The Gram stain has, however, been used on all our cultures, since, in the genus Diplococcus and in many other groups, it has been thought to have such special significance.
1964 M. Hynes Med. Bacteriol. (ed. 8) v. 44 All [bacteria] may be placed into one of two broad groups according to whether they stain by Gram's technique.
2009 B. L. Waters Handbk. Autopsy Pract. (ed. 4) vii. 87/1 There is no reason to culture acute perforations of bowel since both Gram stain and culture will point to fecal flora.
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