1885 Brit. Med. Jrnl. 10 Jan. 91/1 Dr. Klein..criticises the results as to attenuation of virus by sub-cultures, claimed by M. Pasteur.

1899 T. C. Allbutt et al. Syst. Med. VII. 550 Growth..in subcultures may be recognisable within four hours.

1911 Jrnl. Pathol. & Bacteriol. 15 94 In subculture it grew on plain agar.

1953 R. W. Fairbrother Text-bk. Bacteriol. (ed. 7) i. 6 The growth of the organisms in culture was observed, and after several subcultures the organism was still found to be virulent for the mouse.

2006 M. F. Pera et al. in E. Notarianni & M. J. Evans Embryonic Stem Cells x. 241 We recommend that subculture be carried out by mechanical dissection of the colony under a stereo dissecting microscope.

1914 Man 14 195 A matrilineal system of inheritance was a feature of the sub-culture of the south, on which the Brahmanic super-culture was imposed.

1936 R. Linton Study of Man xvi. 275 While ethnologists have been accustomed to speak of tribes and nationalities as though they were the primary culture-bearing units, the total culture of a society of this type is really an aggregate of sub-cultures.

1948 T. S. Eliot Notes Def. Culture iv. 75 We may find ourselves led to the conclusion, that every sub-culture is dependent upon that from which it is an offshoot.

1955 Soc. Forces 34 113 It is difficult to say that the Far West has developed special values, but..its regional subculture has given a special ‘twist’ to many of the universal American values.

1963 Public Policy 12 192 To the extent that the policeman feels the need to develop a police ‘sub-culture’ or ‘code’ different from that of civilians he can be said to be alienated.

1976 M. Deakin & J. Willis Johnny go Home v. 82 The [social] workers dress like their clients... Only their accents betray them as not being part of the sub-culture they are ministering to.

1983 Time 21 Mar. 72/1 This subculture, nicknamed hip hop, is about..status and competition, particularly among males.

1987 J. Rule Memory Board ix. 153 Reading about a homosexual subculture to understand me is insulting.

1991 D. Gaines Teenage Wasteland x. 242 Until the Great Crack Epidemic stimulated our interest in autonomous youth subcultures, American kids were virtually invisible.

2002 E. White Fast Girls x. 189 Madeline and Margaret are denizens of the Goth subculture—fans of Nine Inch Nails, Anne Rice novels, dressing in corsets and fishnets.

1896 Brit. Med. Jrnl. 19 Dec. 1797/1 In applying each of the above tests of ‘copiousness’ various colonies chosen indiscriminately were subcultured.

1949 H. W. Florey in H. W. Florey et al. Antibiotics I. i. 18 This contaminating organism..was subcultured.

1967 M. E. Hale Biol. Lichens i. 8 Ideally the algae should be isolated and subcultured.

2009 W. G. Hopkins & N. P. A. Hüner Introd. Plant Physiol. (ed. 4) xx. 345/1 The new shoots can be separated and sub-cultured to produce more axillary shoots.

1901 Brit. Med. Jrnl. 26 Jan. 243/1 The authors thought there was every probability that the agency which proved fatal to the subcultured bacilli would have an equal if not greater effect upon organisms of high virulence immediately derived from the excreta of an enteric fever patient.

1970 Nature 25 July 383/1 Subcultured gonococci were exposed to antiserum alone.

2008 Vet. Jrnl. 175 339/2 A single colony forming unit of the subcultured S[treptococcus] zooepidemicus isolate was picked from the agar plate using a sterile flamed loop.

1899 G. Newman Bacteria viii. 314 It rapidly loses its virulence on solid media, and is said to be non-virulent after three or four sub-culturings.

1930 Forestry 4 66 Sub-culturing was done with small pieces of rhizomorph, and all cultures so made continued to produce rhizomorphs in great abundance.

2004 FEMS Microbiol. Lett. 231 257/1 Moreover, after the second or third subculturing of a few selected colonies, fewer blue colonies appeared or sometimes none at all.