Analysis of the optimal feature set indicated several important factors in determining aptamer-target interactions.
Bi-Qing Li, Yu-Chao Zhang, Guo-Hua Huang, Wei-Ren Cui, Ning Zhang, Yu-Dong Cai 2014, 'Prediction of Aptamer-Target Interacting Pairs with Pseudo-Amino Acid Composition',PLoS ONE10.1371/journal.pone.0086729. Retrieved from PLOS CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)
Tests for activity showed that the aptamer did not impair locomotor activity.
Matthew R. Holahan, Dan Madularu, Erin M. McConnell, Ryan Walsh, Maria C. DeRosa 2011, 'Intra-Accumbens Injection of a Dopamine Aptamer Abates MK-801-Induced Cognitive Dysfunctionin a Model of Schizophrenia', PLoS ONE10.1371/journal.pone.0022239. Retrieved from PLOS CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)
The developed workflow allows generating and examining further appealing ligand-aptamer complexes.
Alexander Eisold, Dirk Labudde 2018, 'Detailed Analysis of 17β-Estradiol-Aptamer Interactions: A Molecular Dynamics SimulationStudy', Moleculeshttp://www.mdpi.com/1420-3049/23/7/1690. Retrieved from DOAJ CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)
We have taken the endeavour to review the available aptamer-based biosensor (aptasensor) for cortisol detection.
Azrul Syafiq Zainol Abidin, Ruslinda A. Rahim, Mohd Khairuddin Md Arshad, Mohd FaudziFatin Nabilah, Chun Hong Voon, Thean-Hock Tang, Marimuthu Citartan 2017, 'Current and Potential Developments of Cortisol Aptasensing towards Point-of-Care Diagnostics(POTC)', Sensorshttp://www.mdpi.com/1424-8220/17/5/1180. Retrieved from DOAJ CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)
In this review, we first present the limitations and notable advances of aptamer selection.
Zhenjian Zhuo, Yuanyuan Yu, Maolin Wang, Jie Li, Zongkang Zhang, Jin Liu, XiaohaoWu, Aiping Lu, Ge Zhang, Baoting Zhang 2017, 'Recent Advances in SELEX Technology and Aptamer Applications in Biomedicine', International Journal of Molecular Scienceshttps://www.mdpi.com/1422-0067/18/10/2142. Retrieved from DOAJ CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)
This aptamer shows a caspase-independent cytotoxic activity on tumor cell lines.
Perayot Pamonsinlapatham, Réda Hadj-Slimane, Françoise Raynaud, Marc Bickle, ClaudineCorneloup, Audrey Barthelaix, Yves Lepelletier, Perrine Mercier, Matthieu Schapira,Jérôme Samson, Anne-Laure Mathieu, Nicolas Hugo, Olivier Moncorgé, Ivan Mikaelian,Sylvie Dufour, Christiane Garbay, Pierre Colas 2008, 'A RasGAP SH3 peptide aptamer inhibits RasGAP-Aurora interaction and induces caspase-independenttumor cell death.', PLoS ONEhttp://europepmc.org/articles/PMC2483412?pdf=render. Retrieved from PLOS CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)
Rapid lysosomal degradation of the aptamer-reporter resulted in separation of the paired fluorochrome-quencher molecules.
Zihua Zeng, Ching-Hsuan Tung, Youli Zu 2014, 'A Cancer Cell-Activatable Aptamer-Reporter System for One-Step Assay of CirculatingTumor Cells', Molecular Therapy: Nucleic Acidshttp://www.sciencedirect.com/science/article/pii/S2162253116303237. Retrieved from DOAJ CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)
Here, the results of efficiency trials of the aptamer are presented.
Elena Zavyalova, Elena Zavyalova, Nadezhda Samoylenkova, Nadezhda Samoylenkova, AlexanderRevishchin, Askar Turashev, Ilya Gordeychuk, Ilya Gordeychuk, Andrey Golovin, AndreyGolovin, Alexey Kopylov, Alexey Kopylov, Galina Pavlova, Galina Pavlova 2017, 'The Evaluation of Pharmacodynamics and Pharmacokinetics of Anti-thrombin DNA AptamerRA-36', Frontiers in Pharmacologyhttp://journal.frontiersin.org/article/10.3389/fphar.2017.00922/full. Retrieved from DOAJ CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)
We identified and purified two aptamer conformers by means of chromatographic techniques.
David Porciani, Giovanni Signore, Laura Marchetti, Paolo Mereghetti, Riccardo Nifosì,Fabio Beltram 2014, 'Two Interconvertible Folds Modulate the Activity of a DNA Aptamer Against TransferrinReceptor', Molecular Therapy: Nucleic Acidshttp://www.sciencedirect.com/science/article/pii/S2162253116302839. Retrieved from DOAJ CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)
In this article we review the latest developments in aptamer microarray technology.
Martin Witt, Johanna-Gabriela Walter, Frank Stahl 2015, 'Aptamer Microarrays—Current Status and Future Prospects', Microarrayshttp://www.mdpi.com/2076-3905/4/2/115. Retrieved from DOAJ CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)