Typically, the substrate is nonfluorescent and converted to a fluorophore through one or more reactions.
The Scientist (2000)
In these assays, proprietary fluorescent phosphopeptide tracers compete with nonfluorescent phosphopeptides generated during kinase reactions for binding to phosphospecific antibodies.
The Scientist (2000)
The reagent changes from nonfluorescent to fluorescent in the presence of detergent-coated proteins(em~590 nm).