Attempts to grow the organism in culture and in embryonated eggs were unsuccessful.
Karrie Rose, Jessica Agius, Jane Hall, Paul Thompson, John-Sebastian Eden, MukeshSrivastava, Brendan Tiernan, Cheryl Jenkins, David Phalen 2017, 'Emergent multisystemic Enterococcus infection threatens endangered Christmas Islandreptile populations', PLOS ONE10.1371/journal.pone.0181240. Retrieved from PLOS CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)
In addition, isorhamnetin decreased the virus titer in ovo using embryonated chicken eggs.
Ahmed Abdal Dayem, Hye Yeon Choi, Young Bong Kim, Ssang-Goo Cho 2015, 'Antiviral Effect of Methylated Flavonol Isorhamnetin against Influenza', PLOS ONE10.1371/journal.pone.0121610. Retrieved from PLOS CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)
Human toxocariasis develops by ingesting of embryonated eggs in contaminated soil.
Wiwanitkit Viroj, Waenlor Weerachit 2004, 'The frequency rate of Toxocara species contamination in soil samples from public yardsin a urban area "Payathai", Bangkok, Thailand', Revista do Instituto de Medicina Tropical de São Paulohttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652004000200011. Retrieved from DOAJ CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)
Higher isolation rates (74.3-83.3%) were obtained after three passages in embryonated chicken eggs.
M.A Orsi, L Doretto Jr, S.C.A Camillo, D Reischak, S.A.M Ribeiro, A Ramazzoti, A.OMendonça, F.R Spilki, M.G Buzinaro, H.L Ferreira, C.W Arns 2010, 'Prevalence of newcastle disease virus in broiler chickens (Gallus gallus) in Brazil',Brazilian Journal of Microbiologyhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200014. Retrieved from DOAJ CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)