Furthermore, exocytotic trafficking was monitored dynamically by real-time high-throughput fluorescence quantitation.
Takeshi Kiyoi, Shuang Liu, Muhammad Novrizal Abdi Sahid, Masachika Shudou, KazutakaMaeyama, Masaki Mogi 2018, 'High-throughput screening system for dynamic monitoring of exocytotic vesicle traffickingin mast cells', PLOS ONE10.1371/journal.pone.0198785. Retrieved from PLOS CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)
However, we know little about their direct effects on the exocytotic process of mast cells.
Asuka Baba, Masahiro Tachi, Yutaka Ejima, Yasuhiro Endo, Hiroaki Toyama, MitsunobuMatsubara, Kazutomo Saito, Masanori Yamauchi, Chieko Miura, Itsuro Kazama 2016, 'Anti-Allergic Drugs Tranilast and Ketotifen Dose-Dependently Exert Mast Cell-StabilizingProperties', Cellular Physiology and Biochemistryhttp://www.karger.com/Article/FullText/438605. Retrieved from DOAJ CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)
To function optimally, cells are strongly dependent on precisely controlled delivery of exocytotic cargo.
Ivar Noordstra, Anna Akhmanova 2017, 'Linking cortical microtubule attachment and exocytosis [version 1; referees: 2 approved]',F1000Researchhttps://f1000research.com/articles/6-469/v1. Retrieved from DOAJ CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)
Receptors released from exocytotic vesicles initially accumulate faster than receptors released from the other two sources.
Le Novère Nicolas, Tolle Dominic P 2010, 'Brownian diffusion of AMPA receptors is sufficient to explain fast onset of LTP',BMC Systems Biologyhttp://www.biomedcentral.com/1752-0509/4/25. Retrieved from DOAJ CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)
In this article, we demonstrate a computational pipeline to help solve this problem, using the exocytotic process as an example.
Wenhai Chen, Wen Zhou, Tian Xia, Xun Gu 2012, 'A computational analysis framework for molecular cell dynamics: case-study of exocytosis.',PLoS ONEhttp://europepmc.org/articles/PMC3394804?pdf=render. Retrieved from PLOS CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)
Membrane capacitance measurements indicated that rapid endocytosis retrieves at least 70% of the exocytotic membrane increase.
Xiangyi Wen, Xiangyi Wen, Grant W. Saltzgaber, Wallace B. Thoreson, Wallace B. Thoreson 2017, 'Kiss-and-Run Is a Significant Contributor to Synaptic Exocytosis and Endocytosis inPhotoreceptors', Frontiers in Cellular Neurosciencehttp://journal.frontiersin.org/article/10.3389/fncel.2017.00286/full. Retrieved from DOAJ CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)
Our super-resolution-imaging results set the stage for studying co-clustering of thechannel with other exocytotic proteins at a single-molecule level.
Julia Sajman, Michael Trus, Daphne Atlas, Eilon Sherman 2017, 'The L-type Voltage-Gated Calcium Channel co-localizes with Syntaxin 1A in nano-clustersat the plasma membrane', Scientific Reportshttp://link.springer.com/article/10.1038/s41598-017-10588-4. Retrieved from DOAJ CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)