It was heterologously expressed in yeast cells and its enzymatic activity was characterized.
Schwab Wilfried, Huang Fong-Chin 2011, 'Cloning and characterization of a 9-lipoxygenase gene induced by pathogen attack fromNicotiana benthamiana for biotechnological application', BMC Biotechnologyhttp://www.biomedcentral.com/1472-6750/11/30. Retrieved from DOAJ CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)
Cen502 was heterologously expressed, purified, and biochemically characterized.
Lin Zhang, Qiang Fu, Wenpeng Li, Bowen Wang, Xiaoyan Yin, Suyao Liu, Zhaonan Xu, QiuhongNiu 2017, 'Identification and characterization of a novel β-glucosidase via metagenomic analysisof Bursaphelenchus xylophilus and its microbial flora', Scientific Reportshttp://link.springer.com/article/10.1038/s41598-017-14073-w. Retrieved from DOAJ CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)
Its over-exploitation has raised the priority of producing these compounds heterologously.
Xia Guangmin, Liu Chang, Zhao Cuizhu, Wang Junfeng, Xiang Fengning 2011, 'Introgression of Swertia mussotii gene into Bupleurum scorzonerifoliumvia somatic hybridization', BMC Plant Biologyhttp://www.biomedcentral.com/1471-2229/11/71. Retrieved from DOAJ CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)
The protein and its intrinsic inhibitory peptide were heterologously expressed, purified and biochemically characterized.
Fuchsbauer Hans-Lothar, Albert Markus, Bleischwitz Marc, Kaldenhoff Ralf 2010, 'Significance of Cuscutain, a cysteine protease from Cuscuta reflexa, in host-parasiteinteractions', BMC Plant Biologyhttp://www.biomedcentral.com/1471-2229/10/227. Retrieved from DOAJ CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)
This study also highlights the need to explore potential secondary effects of long-term expression and activation of heterologously introduced channels.
Devon C. Crawford, Krista L. Moulder, Robert W. Gereau, Gina M. Story, Steven Mennerick 2009, 'Comparative Effects of Heterologous TRPV1 and TRPM8 Expression in Rat HippocampalNeurons', PLoS ONE10.1371/journal.pone.0008166. Retrieved from PLOS CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)
As with acetylcholine induced currents in the heterologously expressed receptors, contractions induced by acetylcholine were antagonized by monepantel.
Melanie Abongwa, Djordje S. Marjanovic, James G. Tipton, Fudan Zheng, Richard J. Martin,Sasa M. Trailovic, Alan P. Robertson 2018, 'Monepantel is a non-competitive antagonist of nicotinic acetylcholine receptors fromAscaris suum and Oesophagostomum dentatum', International Journal for Parasitology: Drugs and Drug Resistancehttp://www.sciencedirect.com/science/article/pii/S2211320717301227. Retrieved from DOAJ CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)
The three recombinant enzymes were heterologously expressed and investigated for their inhibition profiles with sulfonamides and sulfamates.
Sonia Del Prete, Silvia Bua, Fatmah A. S. Alasmary, Zeid AlOthman, Sylvie Tambutté,Didier Zoccola, Claudiu T. Supuran, Clemente Capasso 2019, 'Comparison of the Sulfonamide Inhibition Profiles of the α-Carbonic Anhydrase Isoforms(SpiCA1, SpiCA2 and SpiCA3) Encoded by the Genome of the Scleractinian Coral Stylophorapistillata', Marine Drugshttp://www.mdpi.com/1660-3397/17/3/146. Retrieved from DOAJ CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)
These studies thus provide a system to understand the redox sensitivity of this heterologously synthesized hydrogenase.
Nadya Dragomirova, Patricia Rothe, Stefan Schwoch, Stefanie Hartwig, Constanze Pinske,R. Gary Sawers 2018, 'Insights Into the Redox Sensitivity of Chloroflexi Hup-Hydrogenase Derived From Studiesin Escherichia coli: Merits and Pitfalls of Heterologous [NiFe]-Hydrogenase Synthesis',Frontiers in Microbiologyhttps://www.frontiersin.org/article/10.3389/fmicb.2018.02837/full. Retrieved from DOAJ CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)
Here, we have studied the functional properties of heterologously expressed vbo 3 with a variety of methods.
Simone Graf, Peter Brzezinski, Christoph von Ballmoos 2019, 'The proton pumping bo oxidase from Vitreoscilla', Scientific Reportshttp://link.springer.com/article/10.1038/s41598-019-40723-2. Retrieved from DOAJ CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)