Removal of the chaotropes frequently causes reaggregation and poor recovery of bioactive proteins.
Cleide Mara Rosa da Silva, Rosa Maria Chura-Chambi, Lennon Ramos Pereira, Yraima Cordeiro,Luís Carlos de Souza Ferreira, Ligia Morganti 2018, 'Association of high pressure and alkaline condition for solubilization of inclusionbodies and refolding of the NS1 protein from zika virus', BMC Biotechnologyhttp://link.springer.com/article/10.1186/s12896-018-0486-2. Retrieved from DOAJ CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)
Light transmittance in ultraviolet-visible spectroscopy has been used to quantify the reaggregation by a series of controlled experiments.
Jiacheng Wei, Rasheed Atif, Thuc Vo, Fawad Inam 2015, 'Graphene Nanoplatelets in Epoxy System: Dispersion, Reaggregation, and MechanicalProperties of Nanocomposites', Journal of Nanomaterialshttp://dx.doi.org/10.1155/2015/561742. Retrieved from DOAJ CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)
However, in order to substitute traditional epoxy reinforcements with graphene, there are still some issues like dispersion, homogenization, and reaggregation.
Jiacheng Wei, Rasheed Atif, Thuc Vo, Fawad Inam 2015, 'Graphene Nanoplatelets in Epoxy System: Dispersion, Reaggregation, and MechanicalProperties of Nanocomposites', Journal of Nanomaterialshttp://dx.doi.org/10.1155/2015/561742. Retrieved from DOAJ CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)