Despite its importance, rhabdomere lipid composition has not been established.
Yorka Muñoz, Karen Fuenzalida, Miguel Bronfman, Arnaldo Gatica, Marcelo Sepúlveda,Juan Bacigalupo, Alejandro D Roth, Ricardo Delgado 2013, 'Fatty acid composition of Drosophila photoreceptor light-sensitive microvilli', Biological Researchhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000300010&lng=en&tlng=en. Retrieved from DOAJ CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)
Here we show that chaoptin synergises with crumbs to ensure optimal rhabdomere width.
Nagananda Gurudev, Michaela Yuan, Elisabeth Knust 2014, 'chaoptin, prominin, eyes shut and crumbs form a genetic network controlling the apicalcompartment of Drosophila photoreceptor cells', Biology Openhttp://bio.biologists.org/content/3/5/332. Retrieved from DOAJ CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)
Re-localization from each stage to the rhabdomere likely involves different strategies.
Minh-Ha Lieu, Maximiliano J Vallejos, Emily Michael, Susan Tsunoda 2012, 'Mechanisms underlying stage-1 TRPL channel translocation in Drosophila photoreceptors.',PLoS ONEhttp://europepmc.org/articles/PMC3282777?pdf=render. Retrieved from PLOS CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)
These cells present a highly specialized photosensitive membrane consisting of thousands of microvilli forming a prominent structure termed a rhabdomere.
Yorka Muñoz, Karen Fuenzalida, Miguel Bronfman, Arnaldo Gatica, Marcelo Sepúlveda,Juan Bacigalupo, Alejandro D Roth, Ricardo Delgado 2013, 'Fatty acid composition of Drosophila photoreceptor light-sensitive microvilli', Biological Researchhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000300010&lng=en&tlng=en. Retrieved from DOAJ CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)
The khc mutant showed a progressive mislocalization in the apical domain along the distal-proximal axis during rhabdomere elongation.
Garrett P League, Sang-Chul Nam 2011, 'Role of kinesin heavy chain in Crumbs localization along the rhabdomere elongationin Drosophila photoreceptor.', PLoS ONEhttp://europepmc.org/articles/PMC3117887?pdf=render. Retrieved from PLOS CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)
Moreover, removing one copy of an endoplasmic reticulum chaperone enhances the rhabdomere attachment in rumi mutant animals.
Amanda R Haltom, Tom V Lee, Beth M Harvey, Jessica Leonardi, Yi-Jiun Chen, Yang Hong,Robert S Haltiwanger, Hamed Jafar-Nejad 2014, 'The protein O-glucosyltransferase Rumi modifies eyes shut to promote rhabdomere separationin Drosophila.', PLoS Geneticshttp://europepmc.org/articles/PMC4238978?pdf=render. Retrieved from PLOS CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)
Isolated eyes (500) were homogenized and subjected to a differential centrifugation protocol that generates a fraction enriched in rhabdomere membrane.
Yorka Muñoz, Karen Fuenzalida, Miguel Bronfman, Arnaldo Gatica, Marcelo Sepúlveda,Juan Bacigalupo, Alejandro D Roth, Ricardo Delgado 2013, 'Fatty acid composition of Drosophila photoreceptor light-sensitive microvilli', Biological Researchhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000300010&lng=en&tlng=en. Retrieved from DOAJ CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)