A total of 10 patients of near-triploidy/tetraploidy karyotype were enrolled.
Yang R, Jiang M, Zhao J, Chen H, Gong J, You Y, Song L, Li Z, Li Q 2019, 'Identification of chromosomal abnormalities and genomic features in near-triploidy/tetraploidy-acuteleukemia by fluorescence in situ hybridization', Cancer Management and Researchhttps://www.dovepress.com/identification-of-chromosomal-abnormalities-and-genomic-features-in-ne-peer-reviewed-article-CMAR. Retrieved from DOAJ CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)
No significant tetraploidy was achieved by colchicine application to seedlings.
Suleyman Avci, Metin Tuna, Mehmet Demir Kaya 2019, 'Autotetraploid plant production in endemic Onobrychis elata with colchicine treatments',Journal of Applied Botany and Food Qualityhttps://ojs.openagrar.de/index.php/JABFQ/article/view/10990. Retrieved from DOAJ CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)
However, other evidence argues against the existence of a tetraploidy checkpoint.
Stearns Tim, Wong Connie 2005, 'Mammalian cells lack checkpoints for tetraploidy, aberrant centrosome number, andcytokinesis failure', BMC Cell Biologyhttp://www.biomedcentral.com/1471-2121/6/6. Retrieved from DOAJ CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)
The purpose of this study was to investigate whether and how p53 influences the transformation from tetraploidy to aneuploidy.
Qiyi Yi, Xiaoyu Zhao, Yun Huang, Tieliang Ma, Yingyin Zhang, Heli Hou, Howard J Cooke,Da-Qing Yang, Mian Wu, Qinghua Shi 2011, 'p53 dependent centrosome clustering prevents multipolar mitosis in tetraploid cells.',PLoS ONEhttp://europepmc.org/articles/PMC3208627?pdf=render. Retrieved from PLOS CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)
These results contradicted the hypothesis that p53 functions as the tetraploidy checkpoint and prevents proliferation of tetraploid cells.
Susumu eOhshima, Atsushi eSeyama 2013, 'Establishment of proliferative tetraploid cells from normal human fibroblasts', Frontiers in Oncologyhttp://journal.frontiersin.org/Journal/10.3389/fonc.2013.00198/full. Retrieved from DOAJ CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)
Furthermore, when cells were challenged with conditions causing ancestral cells to evolve aneuploidy, tetraploidy was maintained in the evolved lines.
Yi-Jin Lu, Krishna B S Swamy, Jun-Yi Leu 2016, 'Experimental Evolution Reveals Interplay between Sch9 and Polyploid Stability in Yeast.',PLoS Geneticshttp://europepmc.org/articles/PMC5094715?pdf=render. Retrieved from PLOS CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)
Tetraploidy constitutes an adaptation to stress and an intermediate step between euploidy and aneuploidy in oncogenesis.
Ilio Vitale, Lorenzo Galluzzi, Sonia Vivet, Lisa Nanty, Philippe Dessen, Laura Senovilla,Ken A. Olaussen, Vladimir Lazar, Michelle Prudhomme, Roy M. Golsteyn, Maria Castedo,Guido Kroemer 2007, 'Inhibition of Chk1 Kills Tetraploid Tumor Cells through a p53-Dependent Pathway',PLoS ONE10.1371/journal.pone.0001337. Retrieved from PLOS CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)
This phenotype likely reflects the known role for the mammalian wts counterparts in the tetraploidy checkpoint.
Amanda Simcox, Sayan Mitra, Sharon Truesdell, Litty Paul, Ting Chen, Jonathan P Butchar,Steven Justiniano 2008, 'Efficient genetic method for establishing Drosophila cell lines unlocks the potentialto create lines of specific genotypes.', PLoS Geneticshttp://europepmc.org/articles/PMC2474701?pdf=render. Retrieved from PLOS CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)