| 释义 |
immunoelectrophoresisenUK
im·mu·no·e·lec·tro·pho·re·sis I0051400 (ĭm′yə-nō-ĭ-lĕk′trə-fə-rē′sĭs, ĭ-myo͞o′-)n. The separation and identification of proteins based on differences in electrical charge and reactivity with antibodies.immunoelectrophoresis (ˌɪmjʊnəʊɪˌlɛktrəʊfəˈriːsɪs) n (Medicine) a technique for identifying the antigens in a blood serum, which are separated into fractions by electrophoresisim•mu•no•e•lec•tro•pho•re•sis (ˌɪm yə noʊ ɪˌlɛk troʊ fəˈri sɪs, ɪˌmyu-)
n. a technique for the separation and identification of mixtures of proteins. Thesaurus| Noun | 1. | immunoelectrophoresis - electrophoresis to separate antigens and antibodiescataphoresis, dielectrolysis, electrophoresis, ionophoresis - the motion of charged particles in a colloid under the influence of an electric field; particles with a positive charge go to the cathode and negative to the anode |
TranslationsimmunoelectrophoresisenUK
Immunoelectrophoresis A combination of the techniques of electrophoresis and immunodiffusion used to separate the components of a mixture of antigens and make them visible by reaction with specific antibodies. A medium such as agar is deposited on a convenient base, for example, a microscopic slide. A small well is cut in the medium. A test solution is deposited in the well, and the contained substances are separated by electrophoresis along one axis of the plate. A trough is then cut in the medium parallel to, but at some distance from, the line of the separated substances. The trough is filled with antiserum which contains antibodies to one or more of the separated substances. The antiserum and substances diffuse toward one another and, where they meet, form curvilinear patterns of precipitation. These can be seen directly in clear media or can be visualized after washing out unreacted materials and staining in opaque media. See Immunoassay immunoelectrophoresis[¦im·yə·nō·i‚lek·trə·fə′rē·səs] (immunology) A serological procedure in which the components of an antigen are separated by electrophoretic migration and then made visible by immunodiffusion of specific antibodies. immunoelectrophoresisenUK
Immunoelectrophoresis DefinitionImmunoelectrophoresis, also called gamma globulin electrophoresis, or immunoglobulin electrophoresis, is a method of determining the blood levels of three major immunoglobulins: immunoglobulin M (IgM), immunoglobulin G (IgG), and immunoglobulin A (IgA).PurposeImmunoelectrophoresis is a powerful analytical technique with high resolving power as it combines separation of antigens by electrophoresis with immunodiffusion against an antiserum. The increased resolution is of benefit in the immunological examination of serum proteins. Immunoelectrophoresis aids in the diagnosis and evaluation of the therapeutic response in many disease states affecting the immune system. It is usually requested when a different type of electrophoresis, called a serum protein electrophoresis, has indicated a rise at the immunoglobulin level. Immunoelectrophoresis is also used frequently to diagnose multiple myeloma, a disease affecting the bone marrow.PrecautionsDrugs that may cause increased immunoglobulin levels include therapeutic gamma globulin, hydralazine, isoniazid, phenytoin (Dilantin), procainamide, oral contraceptives, methadone, steroids, and tetanus toxoid and antitoxin. The laboratory should be notified if the patient has received any vaccinations or immunizations in the six months before the test. This is mainly because prior immunizations lead to the increased immunoglobulin levels resulting in false positive results.It should be noted that, because immunoelectrophoresis is not quantitative, it is being replaced by a procedure called immunofixation, which is more sensitive and easier to interpret.DescriptionSerum proteins separate in agar gels under the influence of an electric field into albumin, alpha 1, alpha 2, and beta and gamma globulins. Immunoelectrophoresis is performed by placing serum on a slide containing a gel designed specifically for the test. An electric current is then passed through the gel, and immunoglobulins, which contain an electric charge, migrate through the gel according to the difference in their individual electric charges. Antiserum is placed alongside the slide to identify the specific type of immunoglobulin present. The results are used to identify different disease entities, and to aid in monitoring the course of the disease and the therapeutic response of the patient to such conditions as immune deficiencies, autoimmune disease, chronic infections, chronic viral infections, and intrauterine fetal infections.There are five classes of antibodies: IgM, IgG, IgA, IgE, and IgD.IgM is produced upon initial exposure to an antigen. For example, when a person receives the first tetanus vaccination, antitetanus antibodies of the IgM class are produced 10 to 14 days later. IgM is abundant in the blood but is not normally present in organs or tissues. IgM is primarily responsible for ABO blood grouping and rheumatoid factor, yet is involved in the immunologic reaction to other infections, such as hepatitis. Since IgM does not cross the placenta, an elevation of this immunoglobulin in the newborn indicates intrauterine infection such as rubella, cytomegalovirus (CMV) or a sexually transmitted disease (STD).IgG is the most prevalent type of antibody, comprising approximately 75% of the serum immunoglobulins. IgG is produced upon subsequent exposure to an antigen. As an example, after receiving a second tetanus shot, or booster, a person produces IgG antibodies in five to seven days. IgG is present in both the blood and tissues, and is the only antibody to cross the placenta from the mother to the fetus. Maternal IgG protects the newborn for the first months of life, until the infant's immune system produces its own antibodies.IgA constitutes approximately 15% of the immunoglobulins within the body. Although it is found to some degree in the blood, it is present primarily in the secretions of the respiratory and gastrointestinal tract, in saliva, colostrum (the yellowish fluid produced by the breasts during late pregnancy and the first few days after childbirth), and in tears. IgA plays an important role in defending the body against invasion of germs through the mucous membrane-lined organs.IgE is the antibody that causes acute allergic reactions; it is measured to detect allergic conditions. IgD, which constitutes the smallest portion of the immunoglobulins, is rarely evaluated or detected, and its function is not well understood.PreparationThis test requires a blood sample.AftercareBecause this test is ordered when either very low or very high levels of immunoglobulins are suspected, the patient should be alert for any signs of infection after the test, including fever, chills, rash, or skin ulcers. Any bone pain or tenderness should also be immediately reported to the physician.RisksRisks for this test are minimal, but may include slight bleeding from the blood-drawing site, fainting or feeling lightheaded after venipuncture, or bruising.Normal resultsReference ranges vary from laboratory to laboratory and depend upon the method used. For adults, normal values are usually found within the following ranges (1 mg = approximately 0.000035 oz. and 1 dL = approximately 0.33 oz.):- IgM: 60-290 mg/dL
- IgG: 700-1,800 mg/dL
- IgA: 70-440 mg/dL
Abnormal resultsIncreased IgM levels can indicate Waldenstroöm's macroglobulinemia, a malignancy caused by secretion of IgM at high levels by malignant lymphoplasma cells. Increased IgM levels can also indicate chronic infections, such as hepatitis or mononucleosis and autoimmune diseases, like rheumatoid arthritis.Decreased IgM levels can be indicative of AIDS, immunosuppression caused by certain drugs like steroids or dextran, or leukemia.Increased levels of IgG can indicate chronic liver disease, autoimmune diseases, hyperimmunization reactions, or certain chronic infections, such as tuberculosis or sarcoidosis.Decreased levels of IgG can indicate Wiskott-Aldrich syndrome, a genetic deficiency caused by inadequate synthesis of IgG and other immunoglobulins. Decreased IgG can also be seen with AIDS and leukemia.Increased levels of IgA can indicate chronic liver disease, chronic infections, or inflammatory bowel disease.Decreased levels of IgA can be found in ataxia, a condition affecting balance and gait, limb or eye movements, speech, and telangiectasia, an increase in the size and number of the small blood vessels in an area of skin, causing redness. Decreased IgA levels are also seen in conditions of low blood protein (hypoproteinemia), and drug immunosuppression.ResourcesBooksFischbach, Frances T. A Manual of Laboratory Diagnostic Tests. Philadelphia: Lippincott Williams & Wilkins, 1999.Pagana, Kathleen D., and Timothy J. Pagana. Mosby's Manual of Diagnostic and Laboratory Tests. St. Louis, MO: Mosby, Inc., 1999.Key termsAntibody — A protein manufactured by the white blood cells to neutralize an antigen in the body. In some cases, excessive formation of antibodies leads to illness, allergy, or autoimmune disorders.Antigen — A substance that can cause an immune response, resulting in production of an antibody, as part of the body's defense against infection and disease. Many antigens are foreign proteins not found naturally in the body, and include germs, toxins, and tissues from another person used in organ transplantation.Autoimmune disorder — A condition in which antibodies are formed against the body's own tissues; for example, in some forms of arthritis.immunoelectrophoresis [im″u-no-e-lek″tro-fo-re´sis] a method of distinguishing proteins and other materials on the basis of their electrophoretic mobility and antigenic specificities.rocket immunoelectrophoresis electrophoresis in which antigen migrates from a well through agar gel containing antiserum, forming cone-shaped (rocket) precipitin bands.im·mu·no·e·lec·tro·pho·re·sis (im'yū-nō-ē-lek'trō-fō-rē'sis), A kind of precipitin test in which the components of one group of immunologic reactants (usually a mixture of antigens) are first separated on the basis of electrophoretic mobility in agar or other medium, the separated components then being identified, by means of the technique of double diffusion, on the basis of precipitates formed by reaction with components of the other group of reactants (antibodies).immunoelectrophoresis (ĭm′yə-nō-ĭ-lĕk′trə-fə-rē′sĭs, ĭ-myo͞o′-)n. The separation and identification of proteins based on differences in electrical charge and reactivity with antibodies.im·mu·no·e·lec·tro·pho·re·sis (im'yū-nō-ĕ-lek'trō-fŏr-ē'sis) A kind of precipitin test in which the components of one group of immunologic reactants are first separated on the basis of electrophoretic mobility; the separated components then being identified on the basis of precipitates formed by reaction with components of the other group of reactants. immunoelectrophoresis the use of ANTIBODY-ANTIGEN reactions to identify PROTEINS that have been separated by ELECTROPHORESIS.im·mu·no·e·lec·tro·pho·re·sis (im'yū-nō-ĕ-lek'trō-fŏr-ē'sis) Precipitin test in which the components of one group of immunologic reactants are first separated on the basis of electrophoretic mobility. See IEP
See IEPimmunoelectrophoresisenUK
Words related to immunoelectrophoresisnoun electrophoresis to separate antigens and antibodiesRelated Words- cataphoresis
- dielectrolysis
- electrophoresis
- ionophoresis
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