immunofluorescence

im·mu·no·fluo·res·cence

I0051450 (ĭm′yə-nō-flo͝o-rĕs′əns, -flô-, -flō-)n. Any of various techniques that use antibodies chemically linked to a fluorescent dye to identify or quantify antigens in a tissue sample.

im′mu·no·fluo·res′cent adj.

immunofluorescence

(ˌɪmjʊnəʊflʊəˈrɛsəns) or

immunoflorescence

n (Medicine) a method used to determine the location of antibodies or antigens in which the antibodies or antigens are labelled with a fluorescent dye

im•mu•no•fluo•res•cence

(ˌɪm yə noʊ flʊˈrɛs əns, -flɔ-, -floʊ-, ɪˌmyu-)

n. any of various techniques for detecting an antigen or antibody in a sample by coupling its specifically interactive antibody or antigen to a fluorescent compound, mixing with the sample, and observing the reaction under an ultraviolet-light microscope. [1955–60]

Thesaurus

Noun

immunofluorescence - (immunology) a technique that uses antibodies linked to a fluorescent dye in order to study antigens in a sample of tissuetechnique - a practical method or art applied to some particular taskimmunology - the branch of medical science that studies the body's immune system

Translations

Immunofluorescence

A technique that uses a fluorochrome to indicate the occurrence of a specific antigen-antibody reaction. The fluorochrome labels either an antigen or an antibody. The labeled reactant is then used to detect the presence of the unlabeled reactant. The use of a labeled reactant (such as an antibody which both detects and indicates the antigen) to reveal the presence of an unlabeled one is termed direct immunofluorescence. The use of a labeled indicator antibody, which reacts with an unlabeled detector antibody that has previously reacted with an antigen, is termed indirect immunofluorescence. Substitution of a light meter for the human eye permits a quantitative measurement in immunofluorometry. See Immunoassay

Immunofluorescence

any set of methods of fluorescent analysis used in immunology, histochemistry, virology, bacteriology, mycology, and parasitology.

The combination of immunochemical reactions with fluorescence microscopy makes possible the detection of tissue and cellular antigens, including those involved in autoimmune diseases and in malignantly degenerated cells. The method is also useful in studying the patterns of antibody synthesis and in identifying the causative agents of many viral and microbial diseases. Specific antibodies are tagged with a fluorescent dye (for example, acridine orange) that will not alter their properties and then introduced to the specimen, so that only the parts of the specimen containing antigen will fluoresce. When the formation of antigen-antibody complexes is being investigated by immuno-fluorescence, the antibodies are tagged with a dye whose fluorescent properties will change when the antibodies combine with antigen.

REFERENCE

Immunofliurestsentsiia. Edited by J. Kubica. Moscow, 1967. (Translated from Polish.)

immunofluorescence

[¦im·yə·nō·flə′res·əns] (immunology) Fluorescence as the result of, or identifying, an immune response; a specifically stained antigen fluoresces in ultraviolet light and can thus be easily identified with a homologous antigen.

immunofluorescence

[im″u-no-floo″o-res´ens] a method of determining the location of antigen (or antibody) in a tissue section or smear using a specific antibody (or antigen) labeled with a fluorochrome" >fluorochrome. There are two major types of immunofluorescence techniques, both based on the reaction" >antigen--antibody reaction, in which the antibody attaches itself to a specific antigen.
In the direct fluorescent antibody (DFA) method, the antibody coats the antigen, for example, a bacterial cell, and cannot be easily removed by elution (washing). The antibody remains attached to the cell after all nonantibody globulin has been washed away. Since the antibody has been rendered fluorescent by conjugation with fluorescein or another dye, the outline of the bacterial cell that it coats can readily be seen with a special microscope.
In the indirect fluorescent antibody (IFA) method, the specific antibody is allowed to react with the antigen. The nonantibody globulin is then washed off. This is then treated with a labeled antibody to the specific antibody. For example, if the specific antibody was raised in a rabbit, it is then treated with fluorescein-labeled anti-rabbit globulin, which results in a combination of this labeled antibody with the rabbit immunoglobulin already attached to the antigen.
Fluorescent antibody studies have been used in the detection of numerous bacterial, viral, fungal, and protozoan infections and in the identification of many microscopic tissue constituents.

Direct immunofluorescence. In direct immunofluorescence the object is visualized using a fluorescein-tagged antibody. From Hart and Shears, 1997.

im·mu·no·fluor·es·cence

(im'yū-nō-flōr-es'ens, i-myū'nō-), An immunohistochemical technique using labeling of antibodies by a fluorescent dye to identify antigenic material specific for the labeled antibody. Specific binding of antibody can be determined microscopically through the production of a characteristic visible light by the application of ultraviolet rays to the preparation.
See also: fluorescent antibody technique.

immunofluorescence

(ĭm′yə-nō-flo͝o-rĕs′əns, -flô-, -flō-)n. Any of various techniques that use antibodies chemically linked to a fluorescent dye to identify or quantify antigens in a tissue sample.

im′mu·no·fluo·res′cent adj.

im·mu·no·fluor·es·cence

(im'yū-nō-flōr-es'ĕns) An immunohistochemical technique using labeling of antibodies by fluorescent dyes to identify bacterial, viral, or other antigenic material specific for the labeled antibody; the binding of antibody can be determined microscopically by the application of ultraviolet rays to the preparation.
See also: fluorescent antibody technique

immunofluorescence

The detection and identification of antigenic material by observing, under the microscope, the fluorescence of known, specific, fluorescein-linked (conjugated) antibodies that have become attached to it.

immunofluorescence

See FLUORESCENT ANTIBODY TECHNIQUE.

immunofluorescence

im·mu·no·fluo·res·cence

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immunoflorescence

im•mu•no•fluo•res•cence

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Immunofluorescence

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Words related to immunofluorescence

noun (immunology) a technique that uses antibodies linked to a fluorescent dye in order to study antigens in a sample of tissue

Related Words