释义 |
melting temperature
melting temperature[′melt·iŋ ‚tem·prə·chər] (biochemistry) The temperature at which denaturing occurs for half of the double helices of deoxyribonucleic acid. melting temperature
tem·per·a·ture mid·point (Tm, tm), the midpoint in the change in optic properties (absorbance, rotation) of a structured polymer (for example, DNA) with increasing temperature. Synonym(s): melting temperaturemelting temperature The point in a DNA hybridisation reaction at which 50% of the nucleotides are annealed (linked) to their complement (i.e., are double-stranded), which is a function of the stringency of the hybridising conditions. |