| 释义 |
DictionarySeeligaseligase chain reaction
ligase [li´gās, lig´ās] any of a class of enzymes that catalyze the joining together of two molecules coupled with the breakdown of a pyrophosphate bond in ATP or a similar triphosphate.DNA ligase either of two enzymes that join two double-helical molecules of DNA together to make a longer DNA molecule.ligase chain reaction a type of amplification" >DNA amplification that uses ligase" >DNA ligase to link chains and amplify the template containing the sequence in question.ligase chain reactiona technique for target amplification of DNA in which DNA ligase is used to join two complementary oligonucleotide probes that have bound to a target sequence in vitro. The ligation product is used as a template for ligation of complementary oligonucleotides that, through repeated enzymatic processing, allow for logarithmic accumulation of products that can be used to determine the presence of the target of interest.ligase chain reaction Ligation amplification reaction Molecular biology A DNA amplification technique for detecting minimal amounts of a known DNA sequence, similar in principle to PCR. See PCR. li·gase chain re·ac·tion (lī'gās chān rē-ak'shŭn) A technique for target amplification of DNA in which DNA ligase is used to join two complementary oligonucleotide probes that have bound to a target sequence in vitro. The ligation product is used as a template for ligation of complementary oligonucleotides that, through repeated enzymatic processing, allow for logarithmic accumulation of products that can be used to determine the presence of the target of interest. AcronymsSeeLCR |