mercaptoethanol agglutination inhibition test
mercaptoethanol agglutination inhibition test
A test that differentiates between IgG and IgM as the immunoglobulin responsible for an agglutination reaction.Mechanism
The MAIT is based on mercaptoethanol’s disrupting the disulfide bonds needed to maintain IgM as a 5-molecule complex; IgM antibody agglutination does not occur after mercaptoethanol treatment, while IgG agglutination remains intact.