| Acronym | Definition |
|---|
| RLS➣Release |
| RLS➣Restless Leg Syndrome |
| RLS➣La Sierra (Amtrak station code; La Sierra, CA) |
| RLS➣Real Life Support (various locations) |
| RLS➣Robert Louis Stevenson |
| RLS➣Rapid Light System (various companies) |
| RLS➣Recursive Least Squares (a mathematical algorithm) |
| RLS➣Rappresentante Dei Lavoratori Per La Sicurezza (Italian: Representative for Job Safety) |
| RLS➣Resource Locator Service |
| RLS➣Record Level Sharing |
| RLS➣Route Listening Service |
| RLS➣Record Level Subscriber |
| RLS➣Recursive Least Means Squares |
| RLS➣Replica Location Service |
| RLS➣Registered Land Surveyor |
| RLS➣Reweighted Least Squares (mathematics) |
| RLS➣Record-Level Sharing (Data Facility Storage Management Subsystem) |
| RLS➣Resource List Server |
| RLS➣Resonance Light Scattering (spectroscopy) |
| RLS➣Rate Limiting Step |
| RLS➣Real Life Soap (Wiki) |
| RLS➣Residence Life Staff |
| RLS➣Rod Lift System (various companies) |
| RLS➣Rural Library Sustainability (project; Bill and Melinda Gates Foundation) |
| RLS➣Received Line Signal |
| RLS➣Root Lateral Spread |
| RLS➣Radiolocation Service |
| RLS➣Remote Library Service |
| RLS➣Real Life Scenario |
| RLS➣Renaissance Lawyer Society |
| RLS➣Road Locomotive Society (UK) |
| RLS➣Resource Loaded Schedule |
| RLS➣Radar Line of Sight |
| RLS➣Return to Launch Site (also seen as RTLS) |
| RLS➣Rail Load Simulator |
| RLS➣Residential Living Supervisor |
| RLS➣Residual Life of the System |
| RLS➣Royal Leamington Sparklers (cheerleading group; UK) |