| 释义 |
spoligotyping spoligotyping (spol?i-go-tip'ing) [Fm. sp(acer) oligtyping] The use of the polymerase chain reaction to identify pathogens, such as Mycobacterium tuberculosis, in laboratory specimens. It relies on the detection of unique spans of repeated DNA sequences found between the active genes of the pathogen. Culture-based methods of identifying mycobacteria are slow, often taking as long as 4 to 6 weeks to identify microorganisms in sputum or blood. DNA fingerprinting requires considerable technical expertise. Spoligotyping is simpler, more economical, and a more efficient means of identifying slowly growing microorganisms. Synonym: spacer oligotyping |