Substrate Phosphorylation

in biochemistry, the synthesis of energy-rich phosphorus compounds using the energy of the oxidation-reduction reactions of glycolysis, which are catalyzed by phosphoglyceraldehyde dehydrogenase and enolase. It also takes place upon oxidation of α-ketoglutaric acid in the Krebs cycle (under the action of a-ketoglutarate dehydrogenase and succinate thiokinase). For bacteria, cases of substrate phosphorylation upon oxidation of pyruvic acid have been described.

Substrate phosphorylation, in contrast to phosphorylation in an electron transport system (seeOXIDATIVE PHOSPHORYLATION), is not inhibited by general poisons, such as dinitrophenol, and is not related to the fixation of enzymes in mitochondrial membranes. The contribution of substrate phosphorylation to the cellular supply of adenosine triphosphate under aerobic conditions is significantly less than the contribution of phosphorylation in an electron transport system.