pulsed-field gel electrophoresis


pulse-field gel e·lec·tro·pho·re·sis

gel electrophoresis in which, after electrophoretic migration has begun, the current is briefly stopped and reapplied in a different orientation; allows for the purification of long DNA molecules. Synonym(s): pulsed-field gel electrophoresis

pulsed-field gel electrophoresis (PFGE)

GEL ELECTROPHORESIS in which the gel is subjected to electric fields alternating between different angles. This allows large DNA MOLECULES, such as complete chromosomes, to become reorientated with the change in electric field, so that they can migrate, in a snake-like manner, through the gel for separation.