sickling test


sickling

 [sik´ling] the development of sickle cells in the blood.sickling test a method of demonstrationg hemoglobin S and the sickling phenomenon in erythrocytes, performed by reducing the oxygen concentration to which the erythrocytes are exposed.

sickle cell test

A qualitative screening test for sickling haemoglobins, especially HbS. In the SCT, sodium metabisulfite or sodium dithionate is used to desolubilise haemoglobin S to the crystallised deoxygenated form, which causes the abnormal RBCs to sickle, as detected by light microscopy.

sickling test

A test that measures the propensity of red blood cells to sickle under conditions of reduced oxygen tension. The test may be performed by adding sodium metabisulfite to a drop of blood and examining the blood smear microscopically. Hemoglobin electrophoresis is an alternative test for sickle cell disease.

sickling test

The observation of a blood film, mixed with a solution of sodium metabisulphite, under the microscope. If haemoglobin S is present the red cells will assume a sickle shape within 20 minutes. See also SICKLE CELL ANAEMIA.