| 释义 |
Western blot analysis
Western blot analysisn. A technique for identifying specific proteins in a mixture, in which the proteins are separated by electrophoresis, transferred to a membrane of nitrocellulose or another material, and identified by their reaction with labeled antibodies.
[Playful coinage after Southern blot analysis.]EncyclopediaSeeblotWestern blot analysis
West·ern blot a·nal·y·sisa procedure in which proteins separated by electrophoresis in polyacrylamide gels are transferred (blotted) onto nitrocellulose or nylon membranes and identified by specific complexing with antibodies that are either pretagged or posttagged with a labeled secondary protein.
See also: immunoblot. [coined to distinguish it from eponymic Southern blot a.] Western blot analysisn. A technique for identifying specific antibodies or proteins in which proteins are separated by electrophoresis, transferred to nitrocellulose, and reacted with antibody.West·ern blot a·nal·y·sis (wes'tĕrn blot ă-nal'i-sis) A procedure in which proteins separated by electrophoresis in polyacrylamide gels are transferred (blotted) onto nitrocellulose or nylon membranes and identified by specific complexing with antibodies that are either pre- or post-tagged with a labeled secondary protein.
See also: immunoblot
Synonym(s): Western blot, Western blotting. [coined to distinguish it from eponymic Southern blot a.]West·ern blot a·nal·y·sis (wes'tĕrn blot ă-nal'i-sis) A procedure in which proteins separated by electrophoresis in polyacrylamide gels are transferred (blotted) onto nitrocellulose or nylon membranes and identified by specific complexing with antibodies that are either pre- or posttagged with a labeled secondary protein.
Synonym(s): Western blot, Western blotting. |