| 释义 |
counterimmunoelectrophoresis
counterimmunoelectrophoresis[¦kau̇nt·ər¦im·yə·nō·i‚lek·trō·fə′r ē·səs] (immunology) Immunoelectrophoresis which uses two wells of application, one above the other, along the electrical axis—the anodal well filled with antibody and the cathodal with a negatively charge antigen; electrophoresis results in the antigen and antibody migrating cathodally and anodally, respectively, and a line of precipitation appears where the two meet. counterimmunoelectrophoresis
counterimmunoelectrophoresis (CIE) [kown″ter-im″u-no-e-lek″tro-fo-re´sis] a laboratory technique in which an electric current is used to accelerate the migration of antibody and antigen through a buffered diffusion medium. Antigens in a gel medium in which the pH is controlled are strongly negatively charged and will migrate rapidly across the electric field toward the anode. The antibody in such a medium is less negatively charged and will migrate in an opposite or “counter” direction toward the cathode. If the antigen and antibody are specific for each other, they combine and form a distinct precipitin line.
The technique of CIE was first applied clinically in 1970 to detect hepatitis B antigen. With modification and refinement it is becoming increasingly useful as a means of detecting antigens or antibodies specific for a variety of infectious diseases. It can be especially valuable as an aid to accurate diagnosis of clinical bacterial infections and the selection of specific therapeutic agents for control of infections once the causative organisms are identified.count·er·im·mu·no·e·lec·tro·pho·re·sis (CIE), (kown'ter-im'yū-nō-ĕ-lek'trō-fōr-ē'sis), A modification of immunoelectrophoresis in which antigen (for example, serum containing hepatitis B virus) is placed in wells cut in the sheet of agar gel toward the cathode and antiserum is placed in wells toward the anode; antigen and antibody, moving in opposite directions, form precipitates in the area between the cells where they meet in concentrations of optimal proportions.count·er·im·mu·no·e·lec·tro·pho·re·sis (kown'tĕr-im'yū-nō-ĕ-lek'trō-fŏr-ē'sis) Immunoelectrophoresis in which antigen is placed in wells cut in the sheet of agar gel toward the cathode, and antiserum is placed in wells toward the anode; antigen and antibody, moving in opposite directions, form precipitates in the area between the cells where they meet in concentrations of optimal proportions. AcronymsSeeCIÉ |