allele-specific PCR

The amplification of specific alleles, or DNA sequence variants, at the same locus by polymerase chain reaction. Specificity is achieved by designing one or both PCR primers so that they partially overlap the site of sequence difference between the amplified alleles.
AS-PCR is two-step nested PCR technique that allows detection of DNA with a point mutation in the presence of a 103–105-fold excess of normal DNA. AS-PCR is based on the observation that Taq polymerase will extend primers with a 3’ mismatch 103 to 106-fold less efficiently than perfectly matched primers; by choosing a primer that is complementary at its 3’ end to a point mutation, selective amplification of a mutant allele can be achieved.

单词	allele-specific pcr
释义	allele-specific PCR allele-specific PCR The amplification of specific alleles, or DNA sequence variants, at the same locus by polymerase chain reaction. Specificity is achieved by designing one or both PCR primers so that they partially overlap the site of sequence difference between the amplified alleles. AS-PCR is two-step nested PCR technique that allows detection of DNA with a point mutation in the presence of a 103–105-fold excess of normal DNA. AS-PCR is based on the observation that Taq polymerase will extend primers with a 3’ mismatch 103 to 106-fold less efficiently than perfectly matched primers; by choosing a primer that is complementary at its 3’ end to a point mutation, selective amplification of a mutant allele can be achieved.
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