cDNA cloning

a cloning technique in which mRNA is first converted to cDNA before insertion into a CLONING VECTOR. cDNA cloning is a particularly useful technique to employ when cloning eukaryotic genes containing INTRONS in BACTERIA. If the GENE is cloned directly in bacteria, the introns would inhibit its EXPRESSION. However, the mRNA, from which the cDNA derives, does not contain introns since these are removed during its formation. Thus cDNA will also lack introns and so its expression will not be prevented in bacteria.

单词	cdna cloning
释义	cDNA cloning cDNA cloning a cloning technique in which mRNA is first converted to cDNA before insertion into a CLONING VECTOR. cDNA cloning is a particularly useful technique to employ when cloning eukaryotic genes containing INTRONS in BACTERIA. If the GENE is cloned directly in bacteria, the introns would inhibit its EXPRESSION. However, the mRNA, from which the cDNA derives, does not contain introns since these are removed during its formation. Thus cDNA will also lack introns and so its expression will not be prevented in bacteria.
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