| 释义 |
DictionarySeevectorcloning vector
cloning vector[′klōn·iŋ ‚vek·tər] (cell and molecular biology) A carrier, such as a bacterial plasmid or bacteriophage, used to insert a genetic sequence, such as a deoxyribonucleic acid fragment or a complete gene, into a host cell such that the foreign genetic material is capable of replication. cloning vector
clon·ing vec·toran autonomously replicating plasmid or phage with regions that are not essential for its propagation in bacteria and into which foreign DNA can be inserted; this foreign DNA is replicated and propagated as if it were a normal component of the vector.A recombinant DNA molecule containing sequences from 2 or more sources—e.g., plasmids, bacteriophage, cosmids—into which foreign DNA—40+ kb—may be inserted for cloning; a DNA sequence capable of self-replication in a suitable host cell, which has site(s) for inserting DNA fragments by recombinant DNA techniques, and genetic markers that allow selection for the vector in a host cell; CVs usually have a selective marker—e.g., for antibiotic resistance—and a site for cleavage by a restriction endonucleaseclon·ing vec·tor (klōn'ing vek'tŏr) An autonomously replicating plasmid or phage with regions that are not essential for its propagation in bacteria and into which foreign DNA can be inserted; this foreign DNA is replicated and propagated as if it were a normal component of the vector. cloning vector see VECTOR. |