| Acronym | Definition |
|---|
| DOTA➣Defense of the Ancients (gaming) |
| DOTA➣Depending on the Artist (trope) |
| DOTA➣Doscientos Ocho Transporte Automotor (Buenos Aires bus line; Buenos Aires, Argentina) |
| DOTA➣Download over the Air (Wavecom Wireless) |
| DOTA➣Declaration of the Americas on Diabetes |
| DOTA➣Département d'Optique Théorique et Appliquée (French: Theoretical and Applied Optics Department) |
| DOTA➣Delaware Occupational Therapy Association |
| DOTA➣Department of Transportation, Airports Division (Hawaii) |