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单词 elute
释义

Definition of elute in English:

elute

verbɪˈl(j)uːtəˈlut
[with object]Chemistry
  • Remove (an adsorbed substance) by washing with a solvent, especially in chromatography.

    these protein bands were eluted and used as immunogen
    Example sentencesExamples
    • After extensive washing of the unbound protein with 40 mM imidazole, the GHS protein was eluted with 250 mM imidazole.
    • After repeated washing captured proteins were eluted from the resin using 10 mM DTT.
    • The clear supernatant was loaded onto a Ni-chelating column, and bound protein was eluted with 500 mM imidazole.
    • Bound material was then eluted with 0.2 M methyl mannoside in the same buffer.
    • A radioactive compound identified as p-coumaroyl ß-glucosyl ester was eluted and stored frozen.

Derivatives

  • elution

  • noun ɪˈl(j)uːʃ(ə)nəˈluʃ(ə)n
    Chemistry
    • The imidazole concentration at elution was 112 mM.
      Example sentencesExamples
      • A linear gradient from 100% A to 100% B during the first 12 min was followed by an isocratic elution for 6 min.
      • In this work we have measured protein interactions using self-interaction chromatography, in which protein is immobilized on chromatographic particles and the retention of the same protein is measured in isocratic elution.
      • These include the single cell gel electrophoresis assay, the gel electrophoresis assay, the alkaline elution and unwinding assays and the analysis of DNA adducts.
      • After separation by TLC and elution from the silica gel, ASG and SG were hydrolysed with 2 N methanolic HCl under reflux for 4 h at 100°C.

Origin

1920s: from Latin elut- 'washed out', from the verb eluere, suggested by German eluieren.

 
 

Definition of elute in US English:

elute

verbəˈlutəˈlo͞ot
[with object]Chemistry
  • Remove (an adsorbed substance) by washing with a solvent, especially in chromatography.

    these protein bands were eluted and used as immunogen
    Example sentencesExamples
    • Bound material was then eluted with 0.2 M methyl mannoside in the same buffer.
    • After extensive washing of the unbound protein with 40 mM imidazole, the GHS protein was eluted with 250 mM imidazole.
    • A radioactive compound identified as p-coumaroyl ß-glucosyl ester was eluted and stored frozen.
    • After repeated washing captured proteins were eluted from the resin using 10 mM DTT.
    • The clear supernatant was loaded onto a Ni-chelating column, and bound protein was eluted with 500 mM imidazole.

Origin

1920s: from Latin elut- ‘washed out’, from the verb eluere, suggested by German eluieren.

 
 
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更新时间:2024/12/23 19:36:38