| 释义 |
Definition of Sepharose in English: Sepharosenoun ˈsɛfərəʊzˈsefəˌrōs mass nountrademark A preparation of agarose used as a gel in chromatography, electrophoresis, and other separation techniques. Example sentencesExamples - The mutant protein was immobilized on CNBr-activated Sepharose according to the manufacturer's instruction.
- The purification of VAA started from aqueous extracts of dried mistletoe leaves and also used affinity chromatography on lactosylated Sepharose as the crucial step as previously described.
- One hundred microliters of 10% (w/v) protein-A Sepharose in lysis buffer was added and mixed at 4° for 1 hr.
- Polyclonal anti-RIP1 antibody was purified over a column of recombinant His-tagged proRIP1 coupled to cyanogen-bromide activated Sepharose.
- After 1 hr incubation at room temperature, labeled proteins were recovered by binding to protein A Sepharose and washing extensively in RIPA buffer as well as 100 mM Tris, pH 7.5, supplemented with NP - 40 (nonionic detergent).
Origin 1960s: of unknown origin. Definition of Sepharose in US English: Sepharosenounˈsefəˌrōs trademark A preparation of agarose used as a gel in chromatography, electrophoresis, and other separation techniques. Example sentencesExamples - The mutant protein was immobilized on CNBr-activated Sepharose according to the manufacturer's instruction.
- One hundred microliters of 10% (w/v) protein-A Sepharose in lysis buffer was added and mixed at 4° for 1 hr.
- Polyclonal anti-RIP1 antibody was purified over a column of recombinant His-tagged proRIP1 coupled to cyanogen-bromide activated Sepharose.
- After 1 hr incubation at room temperature, labeled proteins were recovered by binding to protein A Sepharose and washing extensively in RIPA buffer as well as 100 mM Tris, pH 7.5, supplemented with NP - 40 (nonionic detergent).
- The purification of VAA started from aqueous extracts of dried mistletoe leaves and also used affinity chromatography on lactosylated Sepharose as the crucial step as previously described.
Origin 1960s: of unknown origin. |