| 释义 |
Definition of Southern blot in English: Southern blotnoun Biology A procedure for identifying specific sequences of DNA, in which fragments separated on a gel are transferred directly to a second medium on which assay by hybridization may be carried out. Example sentencesExamples - In Southern blots, DNA is cut with restriction enzymes then separated.
- Standard molecular biological procedures, such as Southern blots, were performed as described.
- Columns of horizontal ovals and rectangles indicate lack of hybridization on the Southern blots.
- Standard techniques were used for DNA cloning, restriction enzyme digestion, Southern blot analysis, and DNA sequencing.
- The assay is similar to the Southern blot except that restriction enzyme digestion and denaturation of the mRNA are not needed.
Origin 1970s: named after Edwin M. Southern (born 1938), British biochemist. Definition of Southern blot in US English: Southern blotnoun Biology A procedure for identifying specific sequences of DNA, in which fragments separated on a gel are transferred directly to a second medium on which detection by hybridization may be carried out. Example sentencesExamples - In Southern blots, DNA is cut with restriction enzymes then separated.
- Columns of horizontal ovals and rectangles indicate lack of hybridization on the Southern blots.
- The assay is similar to the Southern blot except that restriction enzyme digestion and denaturation of the mRNA are not needed.
- Standard techniques were used for DNA cloning, restriction enzyme digestion, Southern blot analysis, and DNA sequencing.
- Standard molecular biological procedures, such as Southern blots, were performed as described.
Origin 1970s: named after Edwin M. Southern (born 1938), British biochemist. |