单词	sonicate
释义	sonicate /ˈsɒnɪkeɪt / Biochemistry verb [with object] Subject (a biological sample) to ultrasonic vibration so as to fragment the cells, macromolecules, and membranes: cells were sonicated in an ice bath... The sample was sonicated to clarity in a sonication bath (Ultrawave). The samples were sonicated for 30 min, followed by centrifugation and overnight incubation at 65°C as described above. The samples were sonicated for 12 sec and cell number was analyzed using a Coulter counter. noun A biological sample which has been sonicated.Finally, the sonicate in the third cuvette was additionally treated with DNase for another 1 h at 25 deg C, then Cyan 40 was added and the emission spectrum was recorded.... The sonicate was cleared by centrifugation at 12,000 g for 30 minutes at 4°C. The sonicate was cleared by microcentrifugation for 5 min at 4° and mixed with the anti-HA.11 antibody coupled to Sepharose beads (prewashed three times with lysis buffer). Derivatives sonication noun ... Lipid hydration was coupled with six cycles of freezing, thawing, and sonication, except sonication was omitted in the last cycle. Chromatin was sheared by sonication, and DNA associated with either CLF1 or ORC1 was separately co-immunoprecipitated using an anti-HA antibody. Cells were disrupted by sonication, and soluble recombinant proteins were purified on the TALON metal-affinity resin (Clontech). Origin 1950s: from sonic + -ate2.
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