英语词汇“Southern blot”的英英意思、用法、释义、翻译、读音、例句、短语、词组-英语词典

Southern blot

noun Biology

A procedure for identifying specific sequences of DNA, in which fragments separated on a gel are transferred directly to a second medium on which assay by hybridization may be carried out.Standard techniques were used for DNA cloning, restriction enzyme digestion, Southern blot analysis, and DNA sequencing....

The assay is similar to the Southern blot except that restriction enzyme digestion and denaturation of the mRNA are not needed.
In Southern blots, DNA is cut with restriction enzymes then separated.

Origin

Late 20th century: named after Edwin M. Southern (born 1938), British biochemist.

单词	Southern blot
释义	Southern blot noun Biology A procedure for identifying specific sequences of DNA, in which fragments separated on a gel are transferred directly to a second medium on which assay by hybridization may be carried out.Standard techniques were used for DNA cloning, restriction enzyme digestion, Southern blot analysis, and DNA sequencing.... The assay is similar to the Southern blot except that restriction enzyme digestion and denaturation of the mRNA are not needed. In Southern blots, DNA is cut with restriction enzymes then separated. Origin Late 20th century: named after Edwin M. Southern (born 1938), British biochemist.
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