英语词汇“sticky end”的英英意思、用法、释义、翻译、读音、例句、短语、词组-英语词典

sticky end

noun Biochemistry

An end of a DNA double helix at which a few unpaired nucleotides of one strand extend beyond the other.Such linkers could be joined by DNA ligase onto the DNA to be inserted and the resulting molecule cut with a restriction enzyme to generate sticky ends....

This works because the enzyme will seek out the corresponding sticky ends on the target DNA, in essence cleaving or cutting out a certain section of the DNA.
Here, we have adapted a method we developed previously for the ligation of DNA triangles; we solve the phosphorylation requirement problem by restricting to create phosphorylated sticky ends.

单词	sticky end
释义	sticky end noun Biochemistry An end of a DNA double helix at which a few unpaired nucleotides of one strand extend beyond the other.Such linkers could be joined by DNA ligase onto the DNA to be inserted and the resulting molecule cut with a restriction enzyme to generate sticky ends.... This works because the enzyme will seek out the corresponding sticky ends on the target DNA, in essence cleaving or cutting out a certain section of the DNA. Here, we have adapted a method we developed previously for the ligation of DNA triangles; we solve the phosphorylation requirement problem by restricting to create phosphorylated sticky ends.
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