mac·u·la·tion \ˌmakyəˈlāshən\noun (-s) Etymology: Middle English maculacion, from Latin maculation-, maculatio, from maculatus + -ion-, -io -ion 1.archaic: the act of spotting or staining or the condition of being spotted or stained 2. a.: spot, stain, blemish < indurated maculations on the cheeks > b.: the system or arrangement of spots and markings on an animal or plant