Biopsy, Muscle

Biopsy, Muscle

Synonym/acronym: N/A.

Common use

To assist in diagnosing muscular disease such as Duchenne’s muscular dystrophy as well as other neuropathies and parasitic infections.

Specimen

Muscle tissue or cells.

Normal findings

(Method: Macroscopic and microscopic examination of tissue) No abnormal tissue or cells.

Description

Muscle biopsy is the excision of a muscle tissue sample for microscopic analysis to determine cell morphology and the presence of tissue abnormalities. This test is used to confirm a diagnosis of neuropathy or myopathy and to diagnose parasitic infestation. A biopsy specimen is usually obtained from the deltoid or gastrocnemius muscle after a surgical incision.

This procedure is contraindicated for

  • high alertPatients with bleeding disorders (related to the potential for prolonged bleeding from the biopsy site)

Indications

  • Assist in confirming suspected fungal infection or parasitic infestation of the muscle
  • Assist in diagnosing the cause of neuropathy or myopathy
  • Assist in the diagnosis of Duchenne’s muscular dystrophy

Potential diagnosis

Abnormal findings related to

  • Alcoholic myopathy
  • Amyotrophic lateral sclerosis
  • Duchenne’s muscular dystrophy
  • Fungal infection
  • Myasthenia gravis
  • Myotonia congenita
  • Parasitic infestation
  • Polymyalgia rheumatica
  • Polymyositis

Critical findings

  • Assessment of clear margins after tissue excision
  • Classification or grading of tumor
  • Identification of malignancy

  • It is essential that critical findings be communicated immediately to the requesting health-care provider (HCP). A listing of these findings varies among facilities.

  • Timely notification of a critical finding for lab or diagnostic studies is a role expectation of the professional nurse. The notification processes will vary among facilities. Upon receipt of the critical finding the information should be read back to the caller to verify accuracy. Most policies require immediate notification of the primary HCP, hospitalist, or on-call HCP. Reported information includes the patient’s name, unique identifiers, critical finding, name of the person giving the report, and name of the person receiving the report. Documentation of notification should be made in the medical record with the name of the HCP notified, time and date of notification, and any orders received. Any delay in a timely report of a critical finding may require completion of a notification form with review by Risk Management.

Interfering factors

  • If electromyography is performed before muscle biopsy, residual inflammation may lead to false-positive biopsy results.
  • Failure to follow dietary restrictions before the procedure may cause the procedure to be canceled or repeated.

Nursing Implications and Procedure

Pretest

  • Positively identify the patient using at least two unique identifiers before providing care, treatment, or services.
  • Patient Teaching: Inform the patient this procedure can assist in establishing a diagnosis of musculoskeletal disease.
  • Obtain a history of the patient’s complaints, including a list of known allergens, especially allergies or sensitivities to latex or anesthetics.
  • Obtain a history of the patient’s immune and musculoskeletal systems, any bleeding disorders or other symptoms, and results of previously performed laboratory tests and diagnostic and surgical procedures.
  • Record the date of the last menstrual period and determine the possibility of pregnancy in perimenopausal women.
  • Note any recent procedures that can interfere with test results.
  • Obtain a list of the patient’s current medications including anticoagulants, aspirin and other salicylates, herbs, nutritional supplements, and nutraceuticals (see Effects of Natural Products on Laboratory Values online at DavisPlus). Such products should be discontinued by medical direction for the appropriate number of days prior to a surgical procedure.
  • Review the procedure with the patient. Inform the patient that it may be necessary to remove hair from the site before the procedure. Instruct the patient that prophylactic antibiotics may be administered before the procedure. Address concerns about pain and explain that a sedative and/or analgesia will be administered before the percutaneous biopsy to promote relaxation and reduce discomfort; general anesthesia will be administered before the open biopsy. Explain to the patient that no pain will be experienced during the test when general anesthesia is used but that any discomfort with a needle biopsy will be minimized with local anesthetics and systemic analgesics. Inform the patient that the biopsy is performed under sterile conditions by an HCP specializing in this procedure. The surgical procedure usually takes about 20 min to complete, and sutures may be necessary to close the site. A needle biopsy usually takes about 15 min to complete.
  • Sensitivity to social and cultural issues, as well as concern for modesty, is important in providing psychological support before, during, and after the procedure.
  • Explain that an IV line may be inserted to allow infusion of IV fluids, antibiotics, anesthetics, or sedatives.
  • Instruct the patient that to reduce the risk of nausea and vomiting, solid food and milk or milk products have been restricted for at least 8 hr, and clear liquids have been restricted for at least 2 hr prior to general anesthesia, regional anesthesia, or sedation/analgesia (monitored anesthesia). The American Society of Anesthesiologists has fasting guidelines for risk levels according to patient status. More information can be located at www.asahq.org. Patients on beta blockers before the surgical procedure should be instructed to take their medication as ordered during the perioperative period. Protocols may vary among facilities.
  • Make sure a written and informed consent has been signed prior to the procedure and before administering any medications.

Intratest

  • Potential complications:

  • Bleeding (related to a bleeding disorder, or the effects of natural products and medications known to act as blood thinners)

  • Ensure that the patient has complied with dietary restrictions.
  • Ensure that anticoagulant therapy has been withheld for the appropriate number of days prior to the procedure. Number of days to withhold medication is dependent on the type of anticoagulant. Notify the HCP if patient anticoagulant therapy has not been withheld. Ensure that patients on beta-blocker therapy have continued their medication regimen as ordered.
  • Avoid the use of equipment containing latex if the patient has a history of allergic reaction to latex.
  • Have emergency equipment readily available.
  • Have the patient void before the procedure.
  • Observe standard precautions, and follow the general guidelines in Patient Preparation and Specimen Collection. Positively identify the patient, and label the appropriate specimen containers with the corresponding patient demographics, initials of the person collecting the specimen, date and time of collection, and site location.
  • Assist the patient to a comfortable position: a supine position (for deltoid biopsy) or prone position (for gastrocnemius biopsy). Instruct the patient to cooperate fully and to follow directions. Direct the patient to breathe normally and to avoid unnecessary movement during the local anesthetic and the procedure.
  • Record baseline vital signs, and continue to monitor throughout the procedure. Protocols may vary among facilities.
  • After the administration of general or local anesthesia, use clippers to remove hair from the surgical site if appropriate, cleanse the site with an antiseptic solution, and drape the area with sterile towels.

    • Open Biopsy

  • Adhere to Surgical Care Improvement Project (SCIP) quality measures. Administer ordered prophylactic antibiotics 1 hr before incision, and use antibiotics that are consistent with current guidelines specific to the procedure.
  • After administration of general anesthesia and surgical preparation are completed, an incision is made, suspicious areas are located, and tissue samples are collected.

    • Needle Biopsy

  • Instruct the patient to take slow deep breaths when the local anesthetic is injected. Protect the site with sterile drapes.
  • After infiltration of the site with local anesthetic, a cutting biopsy needle is introduced through a small skin incision and bored into the muscle. A core needle is introduced through the cutting needle, and a plug of muscle is removed. The needles are withdrawn, and the specimen is placed in a preservative solution. Pressure is applied to the site for 3 to 5 min, and then a pressure dressing is applied.

    • General

  • Monitor the patient for complications related to the procedure (e.g., allergic reaction, anaphylaxis).
  • Place tissue samples in properly labeled specimen container containing formalin solution, and promptly transport the specimen to the laboratory for processing and analysis.

Post-Test

  • Inform the patient that a report of the results will be made available to the requesting HCP, who will discuss the results with the patient.
  • Instruct the patient to resume preoperative diet, as directed by the HCP.
  • Monitor vital signs and neurological status every 15 min for 1 hr, then every 2 hr for 4 hr, and then as ordered by the HCP. Monitor temperature every 4 hr for 24 hr. Compare with baseline values. Notify the HCP if temperature is elevated. Discontinue prophylactic antibiotics within 24 hr after the conclusion of the procedure. Protocols may vary among facilities.
  • Observe/assess for delayed allergic reactions, such as rash, urticaria, tachycardia, hyperpnea, hypertension, palpitations, nausea, or vomiting.
  • Observe/assess the biopsy site for bleeding, inflammation, or hematoma formation.
  • Instruct the patient in the care and assessment of the site.
  • Instruct the patient to report any redness, edema, bleeding, or pain at the biopsy site.
  • Assess for nausea and pain. Administer antiemetic and analgesic medications as needed and as directed by the HCP.
  • Administer antibiotic therapy if ordered. Remind the patient of the importance of completing the entire course of antibiotic therapy, even if signs and symptoms disappear before completion of therapy.
  • Recognize anxiety related to test results. Discuss the implications of abnormal test results on the patient’s lifestyle. Provide teaching and information regarding the clinical implications of the test results, as appropriate. Educate the patient regarding access to counseling services.
  • Reinforce information given by the patient’s HCP regarding further testing, treatment, or referral to another HCP. Inform the patient of a follow-up appointment for removal of sutures, if indicated. Answer any questions or address any concerns voiced by the patient or family.
  • Instruct the patient in the use of any ordered medications. Explain the importance of adhering to the therapy regimen. As appropriate, instruct the patient in significant side effects and systemic reactions associated with the prescribed medication. Encourage him or her to review corresponding literature provided by a pharmacist.
  • Depending on the results of this procedure, additional testing may be performed to evaluate or monitor progression of the disease process and determine the need for a change in therapy. Evaluate test results in relation to the patient’s symptoms and other tests performed.

Related Monographs

  • Related tests include AChR, aldolase, ANA, antibody Jo-1, antithyroglobulin antibodies, CK and isoenzymes, EMG, ENG, myoglobin, and RF.
  • Refer to the Immune and Musculoskeletal systems tables at the end of the book for related tests by body system.