| 释义 |
Southern, n.2 Biochem.|ˈsʌðən|
[The name of Edwin M. Southern (b.1938), British biochemist.]
Used attrib. (chiefly in Southern blot, Southern blotting: see *blot n.1 1 f, *blotting vbl. n. 4) with reference to a technique for the identification of specific nucleotide sequences in DNA, in which fragments separated on a gel are transferred directly to a second medium on which assay by hybridization, etc., may be carried out.
1979Proc. Nat. Acad. Sci. LXXVI. 860 (caption) Localization of the V and C regions in Ch603α6 by hybridization to Southern blots. Ibid. 858/2 Filter hybridizations by the Southern blot procedure. 1979Cell XVI. 797/1 Visualization of such higher order bands was achieved by the use of the Southern blotting technique. 1981Anal. Biochem. CXVI. 237/1 In the last 5 years, the Southern blot technique..has become one of the most common techniques in molecular biology. 1984L. W. Browder Developmental Biol. (ed. 2) iii. 100 Southern blotting is used when one needs to detect specific DNA fragments from a large, heterogeneous mixture of fragments, as, for example, following restriction enzyme treatment of DNA. 1990Jrnl. Exper. Bot. XLI. 1047/2 The presence of the NPTII gene in this tissue was confirmed by Southern hybridization of the product. 1991Nature 9 May 119/2 (caption) No band corresponding to Zfy-1 was seen, demonstrating the lack of a Y chromosome; this result was confirmed by Southern blotting using Y-chromosome probes. |