subcloningn.

Brit.

/ˈsʌbkləʊnɪŋ/, U.S.

/ˈsəbˌkloʊnɪŋ/

Origin: Formed within English, by derivation. Etymons: sub- prefix, cloning n.

Etymology: < sub- prefix + cloning n., after subclone n. Compare slightly later subclone v.

Chiefly Genetics.

The production of a subclone; esp. (in later use) the action or process of transferring a fragment of DNA from one vector to another.

ΚΠ

1960 Bull. Hygiene 35 314/1 Additional subcloning of the 6 cell lines in general did not reveal any heterogeneity in sensitivity to poliovirus of the clonal populations.

1985 Proc. National Acad. Sci. U.S.A. 82 3244 The sequencing was largely, though not entirely, completed without subcloning.

2007 J. W. Dale & M. von Schantz From Genes to Genomes (ed. 2) vii. 130 In subcloning, the recombinant insert is transferred to another vector, almost invariably a plasmid, which is more amenable for growth, purification and analysis.

This is a new entry (OED Third Edition, June 2012; most recently modified version published online March 2022).

单词	subcloning
释义	subcloningn. Brit. /ˈsʌbkləʊnɪŋ/, U.S. /ˈsəbˌkloʊnɪŋ/ Origin: Formed within English, by derivation. Etymons: sub- prefix, cloning n. Etymology: < sub- prefix + cloning n., after subclone n. Compare slightly later subclone v. Chiefly Genetics. The production of a subclone; esp. (in later use) the action or process of transferring a fragment of DNA from one vector to another. ΚΠ 1960 Bull. Hygiene 35 314/1 Additional subcloning of the 6 cell lines in general did not reveal any heterogeneity in sensitivity to poliovirus of the clonal populations. 1985 Proc. National Acad. Sci. U.S.A. 82 3244 The sequencing was largely, though not entirely, completed without subcloning. 2007 J. W. Dale & M. von Schantz From Genes to Genomes (ed. 2) vii. 130 In subcloning, the recombinant insert is transferred to another vector, almost invariably a plasmid, which is more amenable for growth, purification and analysis. This is a new entry (OED Third Edition, June 2012; most recently modified version published online March 2022). < n.1960
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